Biology

Microbes in Sewage Treatment

Biology·Revision Notes

Primary and Secondary Treatment — Revision Notes

NEET UG
Version 1Updated 21 Mar 2026

⚡ 30-Second Revision

  • Sewage TreatmentRemoves pollutants from wastewater.
  • Primary TreatmentPhysical process. Removes large solids & grit.

- Steps: Screening ightarrowightarrow Grit Removal ightarrowightarrow Primary Sedimentation. - Output: Primary effluent (liquid), Primary sludge (solids).

  • Secondary Treatment (Biological)Uses microbes to reduce organic matter.

- Steps: Aeration Tank (aerobic microbes, floc formation) ightarrowightarrow Secondary Sedimentation (activated sludge settles). - Aeration Tank: Oxygen supplied, aerobic microbes form flocs, consume organic matter. - Flocs: Masses of bacteria and fungi. - BOD (Biochemical Oxygen Demand): Measure of organic pollution. High BOD = high pollution. Secondary treatment significantly reduces BOD. - Activated Sludge: Settled microbial flocs, partly recycled to aeration tank.

  • Anaerobic Sludge DigesterTreats primary & waste activated sludge.

- Anaerobic bacteria break down organic matter. - Produces Biogas: CH4CH_4 (major), CO2CO_2, H2SH_2S.

  • Treated EffluentWater after secondary treatment, low BOD, safe for discharge.

2-Minute Revision

Sewage treatment is essential to purify wastewater before discharge. It begins with Primary Treatment, a physical process. Here, large debris is removed by screening, followed by grit removal where sand and small stones settle. Then, in primary settling tanks, suspended solids settle to form primary sludge, leaving behind primary effluent. This stage removes about 50-60% of suspended solids and some BOD.

Next is Secondary Treatment, a biological process. The primary effluent enters aeration tanks where air is continuously pumped in. This promotes the growth of aerobic microbes which form flocs (masses of bacteria and fungi).

These microbes consume the dissolved organic matter, significantly reducing the Biochemical Oxygen Demand (BOD), which is a measure of organic pollution. The water then goes to secondary settling tanks, where the microbial flocs settle as activated sludge.

A part of this activated sludge is recycled back to the aeration tank to maintain active microbial populations. The excess primary and activated sludge are sent to anaerobic sludge digesters, where anaerobic bacteria break down the organic matter, producing biogas (mainly methane, carbon dioxide, hydrogen sulfide).

The final treated effluent has a very low BOD and is safe for discharge.

5-Minute Revision

Sewage, or wastewater, is a complex mixture of pollutants that requires extensive treatment to prevent environmental damage and health hazards. The process is broadly divided into primary and secondary treatment stages.

Primary Treatment is the initial, physical stage. Raw sewage first passes through bar screens to remove large floating debris like rags and plastics. Following this, it enters grit chambers where the flow velocity is reduced, allowing heavier inorganic solids such as sand and grit to settle.

The sewage then moves into large primary settling tanks (or clarifiers). Here, suspended organic and inorganic solids settle out by gravity, forming primary sludge at the bottom. Floating materials like oil and grease are skimmed off the surface.

The liquid portion, now called primary effluent, still contains significant dissolved organic matter and microbes. This physical process typically removes 50-60% of suspended solids and 25-35% of the BOD.

Secondary Treatment, also known as biological treatment, is the core purification stage. The primary effluent is transferred to large aeration tanks. In these tanks, air is continuously supplied, creating an aerobic environment.

This oxygen-rich condition is ideal for the rapid growth of beneficial aerobic microorganisms (bacteria, fungi, protozoa). These microbes aggregate to form visible, fluffy masses called flocs.

The flocs actively consume the dissolved organic matter present in the sewage, oxidizing it into simpler, harmless substances like carbon dioxide and water. This microbial activity dramatically reduces the Biochemical Oxygen Demand (BOD) of the wastewater.

BOD is a critical indicator of organic pollution; a high BOD means more oxygen is required by microbes to break down organic matter, indicating higher pollution. After several hours in the aeration tank, the 'mixed liquor' (sewage + flocs) flows into secondary settling tanks.

Here, the microbial flocs settle down, forming activated sludge. A crucial step is the recycling of a portion of this activated sludge back to the aeration tank to maintain a high concentration of active microorganisms, ensuring continuous and efficient treatment.

The remaining excess primary sludge and waste activated sludge are then pumped into anaerobic sludge digesters. In these sealed tanks, anaerobic bacteria break down the complex organic polymers in the sludge.

This anaerobic digestion process produces biogas, a valuable mixture primarily composed of methane (CH4CH_4, 50-75%), carbon dioxide (CO2CO_2, 25-45%), and traces of hydrogen sulfide (H2SH_2S). Methane can be used as a fuel.

The treated water, or secondary effluent, now has a significantly reduced BOD (typically 85-95% reduction) and is generally safe for discharge into natural water bodies, minimizing environmental impact.

Prelims Revision Notes

Primary and Secondary Treatment: NEET Essentials

1. Sewage (Wastewater):

  • Domestic, industrial, agricultural waste.
  • Contains organic matter, pathogens, nutrients, suspended solids.
  • Requires treatment to prevent pollution and disease.

2. Primary Treatment (Physical Process):

  • GoalRemove large and suspended solids.
  • Steps in Sequence:

1. Screening: Removes large floating debris (rags, plastics, sticks) using bar screens. 2. Grit Removal: Sewage velocity reduced in grit chambers; allows heavier inorganic solids (sand, grit) to settle. 3. Primary Sedimentation (Settling Tank): Suspended organic and inorganic solids settle by gravity, forming primary sludge. Floating grease/oil skimmed off.

  • OutputPrimary effluent (liquid) and primary sludge (solids).
  • EffectivenessRemoves ~50-60% suspended solids, ~25-35% BOD.

3. Secondary Treatment (Biological Process - Activated Sludge Process):

  • GoalReduce dissolved organic matter and BOD using microbes.
  • Steps in Sequence:

1. Aeration Tank: Primary effluent is pumped in. Air is continuously supplied (aeration). * Environment: Aerobic (oxygen-rich). * Microbes: Aerobic heterotrophic bacteria and fungi grow rapidly, forming flocs (microbial aggregates).

* Function: Flocs consume dissolved organic matter, oxidizing it to CO2CO_2 and H2OH_2O. This significantly reduces BOD. 2. Secondary Sedimentation (Settling Tank): Aerated mixed liquor flows here.

* Process: Microbial flocs settle by gravity, forming activated sludge. * Sludge Recycling: A portion of activated sludge is pumped back to the aeration tank (inoculum). * Waste Sludge: Excess activated sludge sent for anaerobic digestion.

  • OutputSecondary effluent (liquid, low BOD) and activated sludge (solids).
  • EffectivenessReduces BOD by ~85-95%.

4. Biochemical Oxygen Demand (BOD):

  • DefinitionAmount of dissolved oxygen required by aerobic microorganisms to decompose organic matter in a water sample (usually 5 days at 20circC20^circ C).
  • SignificanceHigher BOD = more organic pollution = greater oxygen demand by microbes = potential oxygen depletion for aquatic life.
  • Treatment ImpactSecondary treatment drastically reduces BOD.

5. Anaerobic Sludge Digestion:

  • LocationLarge, closed anaerobic sludge digesters.
  • InputPrimary sludge + waste activated sludge.
  • MicrobesAnaerobic bacteria.
  • ProcessBreak down complex organic polymers.
  • ProductBiogas (mixture of gases).

* Composition: Primarily Methane (CH4CH_4, 50-75%), Carbon dioxide (CO2CO_2, 25-45%), Hydrogen sulfide (H2SH_2S, traces). * Use: Methane is a fuel, can generate electricity/heat.

6. Final Effluent: After secondary treatment, water is much cleaner, with low BOD, and can be safely discharged.

Vyyuha Quick Recall

To remember the sequence and key aspects of sewage treatment:

Physical Separation Always Starts Aerobic Sludge Digestion

  • Physical: Primary Treatment
  • Separation: Screening & Grit Removal
  • Always: Primary Aeration (Settling)
  • Starts: Secondary Treatment
  • Aerobic: Aeration Tank (Aerobic microbes, Flocs, BOD reduction)
  • Sludge: Secondary Settling (Activated Sludge)
  • Digestion: Anaerobic Sludge Digestion (Biogas)
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