Biology·Prelims Strategy

Regulation of Glycolysis — Prelims Strategy

NEET UG
Version 1Updated 22 Mar 2026

Prelims Strategy

To excel in NEET questions on glycolysis regulation, a multi-pronged strategy is essential. Firstly, memorize the three key regulatory enzymes (Hexokinase/Glucokinase, PFK-1, Pyruvate Kinase) and their respective steps.

Secondly, for each regulatory enzyme, list its primary allosteric activators and inhibitors. Pay special attention to molecules like ATP, AMP, citrate, and Fructose-2,6-bisphosphate, understanding *why* they activate or inhibit (e.

g., ATP signals high energy, so it inhibits catabolism). Thirdly, understand the role of hormonal control, particularly insulin and glucagon, and their specific effects on liver glycolytic enzymes, often involving phosphorylation/dephosphorylation.

Remember that insulin generally promotes glycolysis, while glucagon inhibits it. Fourthly, differentiate between Hexokinase and Glucokinase in terms of KmK_m, tissue distribution, and product inhibition.

Finally, practice conceptual questions that present physiological scenarios (e.g., 'What happens to glycolysis in muscle during intense exercise?') and require you to apply your knowledge of regulatory mechanisms.

Be wary of trap options that swap activators for inhibitors or confuse the regulation of different enzymes.

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